Application of ANOVA for Plant Breeding: Single Marker Analysis Example using ANOVA in a Balanced Population (Sample SAS Program)

This module provides an example of using analysis of variance (ANOVA) to assess differences in tomato bacterial spot resistance due to molecular marker genotype (treatment effect) to determine whether genotype of each molecular marker results in differences in disease severity in a BC₁ population.

The following links provide:

• The example
• The dataset
• The SAS output

SAS Code

The SAS code is presented in two ways:

• As a screenshot taken from SAS after the code has been entered (Fig.1)
• As plain text

Figure 1. SAS Screenshot taken after SAS code for analysis of variance in a balanced population was entered. Screenshot credit: David Francis, The Ohio State University.

Plain Text SAS Code

data map;
     infile ‘a:\lnkspt.csv’; delimiter = “,” firstobs = 4;
     input gen vsc pop tg23 pto;
proc sort;
     by tg23;
proc glm;
     class tg23;
     model pop = tg23;
     means tg23 / lsd lines;
proc sort;
     by pto;
proc glm;
     class pto;
     model pop = pto;
     means pto / lsd lines;
run;

Funding Statement

Development of this page was supported in part by the National Institute of Food and Agriculture (NIFA) Solanaceae Coordinated Agricultural Project, agreement 2009-85606-05673, administered by Michigan State University. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the United States Department of Agriculture.

PBGworks 906