How to Use Doubled Haploids to Improve Winter Wheat


Bill Berzonsky, South Dakota State University; Melanie Caffe-Treml, South Dakota State University; Heather L. Merk, The Ohio State University

This webinar explores the use of doubled haploids to improve winter wheat in the context of the South Dakota State University winter wheat breeding program.

 During this webinar Dr. Bill Berzonsky discusses strategy in applying doubled haploids to winter wheat breeding. Dr Melanie Caffe-Treml describes the doubled haploid technique.

This one hour webinar has been divided into two videos (one for each presenter), which are listed in order on this webpage. The powerpoint slides (in pdf format) are provided at the bottom of the page.

Learning Objectives

At the end of this webinar you should be able to:

  • Describe the advantages and disadvantages of the double haploid technique
  • List the required steps to generate doubled haploids
  • Describe the role of maize in wheat doubled haploid production
  • Calculate the number of florets that should be pollinated to generate a desired number of doubled haploids


Part 1 – Dr. Bill Berzonsky

If you experience problems viewing this video connect to our YouTube channel or see the YouTube troubleshooting guide.

Part 2 – Dr. Melanie Caffe-Treml

If you experience problems viewing this video connect to our YouTube channel or see the YouTube troubleshooting guide.


About the Presenters

Dr. Melanie Caffe-Treml is a Post-Doc Research Associate within the Winter Wheat Breeding project at South Dakota State University. She was hired in January as part of the “Winter Cereals Sustainability in Action (WCSIA)” project funded by Ducks Unlimited and Bayer Crop Science to implement the doubled-haploid technology within the breeding program. She received a Ph.D. in Biological Sciences from South Dakota State University in 2010, a M.S. in Agronomy from the National Institute of Agronomy of Toulouse (ENSAT), France, in 2004, and a B.S. in Cell Biology and Physiology from the University of Sciences and Technology of Lille, France, in 2002.

Dr. Bill Berzonsky is Associate Professor and leader of the South Dakota State University Winter Wheat Breeding Program. He assumed this position in September of 2008. He received a Ph.D. in Agronomy from the University of Missouri in 1988, a M.S. in Plant Sciences from the University of Delaware in 1984, and a B.S. in Botany from the University of Maryland in 1982. As a participant in the WCSIA project, he is charged with implementing breeding techniques and strategies to promote the development of winter wheat varieties for the Prairie Pothole Region of South Dakota and the surrounding areas.

Register for, or watch other plant breeding and genomics “How To” webinars

Funding Statement

Development of this page was supported in part by the National Institute of Food and Agriculture (NIFA) Solanaceae Coordinated Agricultural Project, agreement 2009-85606-05673, administered by Michigan State University. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the United States Department of Agriculture.

PBGworks 1209