Heather L. Merk, The Ohio State University; Allen Van Deynze, University of California, Davis
In the first video clip, Dr. Allen Van Deynze, University of California, Davis, provides an overview of Sanger sequencing, next generation/second generation sequencing, and third generation sequencing technologies. In addition, the sequencing technologies are compared based on the amount of sequence generated, the cost, and the sequence read length.
In the second video clip, Dr. Van Deynze provides visual representations of the portion of the genome sequenced using different sequencing technologies, and he discusses the potential utility of the sequence, from expressed sequence tags (ESTs), to the transcriptome, to the whole genome.
For a review of sequencing technologies,
- Thompson, J. F., and P. M. Milos. 2011. The properties and applications of single molecule sequencing. Genome Biology 12: 217. (Available online at: genomebiology.com/2011/12/2/217) (verified 25 Feb 2011).
Development of this lesson was supported in part by the National Institute of Food and Agriculture (NIFA) Solanaceae Coordinated Agricultural Project, agreement 2009-85606-05673, administered by Michigan State University. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the United States Department of Agriculture.
Mention of specific companies is not intended for promotional purposes.
Next_Gen_Sequencing.pdf (2.3 MB)